Biocatalysis as a Tool for the Synthetic Chemist 2018

Topics

• Hydrolytic enzymes (lipases, esterases, amidases, proteases)
  • Kinetic resolution
  • Mechanism of hydrolysis by enzymes
  • Dynamic kinetic resolution (DKR)
  • Use of organic solvents
  • Ester, amide, nitrile and epoxide hydrolysis
  • Deracemisation
  • Concentration
• Introduction to biocatalysis
  • Myths about biocatalysis / pros and cons of biocatalysis
  • Literature sources for biocatalysis (books and review articles)
  • Sources of biocatalysts
  • Enzyme / protein basics, basic microbiology
  • Enzyme types
  • Equipment requirements
• Reduction
  • Co-factors
  • Ketone reduction, Prelog rules
  • Enantioselective reduction / DKR
  • Deracemisation of secondary alcohols
  • C=C reduction
  • Keto-ester reduction
• Oxidations
  • Amine oxidation, deracemisation of amines and amino acids
  • Alcohol oxidation
  • Demethylation
  • Bio-oxygenation, bio-hydroxylation
  • Bayer-Villiger oxidation
  • Epoxidation
  • Sulphoxidation
• Scale up of biotransformations
  • Case studies
  • Reactors
• C-c and c-x bond formation
  • Transketolases
  • Aldol reactions
  • Hydroxy-nitrile lyases
  • Cross benzoin reactions
  • Transamination
  • Michael reactions
  • Fumarase and aspartase
• Pros and cons of biocatalysis
• New developments including directed evolution
  • Mutagenesis
  • Polymerase Chain Reaction
  • Product isolation
  • Directed Evolution

Who should Attend

Organic Chemists involved in:

• Designing novel
• Synthesis
• Green synthetic routes