10th Brazil School for Single Particle Cryo-EM

The 10th Brazil School for Single Particle Cryo-EM is an hands-on workshop in the field of Cryo-electron microscopy (cryo-EM).

Topics

• Properties of the Fourier transform
• The Fourier transform in 1D - 2D - 3D
• (Automatic) Particle selection
• Specimen preparation for single-particle cryo-EM
• CTF correction
• Optimizing the EM data collection
• Alignment using correlation functions
• Filtering and data normalization
• Multivariate statistics and classification
• Multi-reference alignments
• Three-dimensional reconstruction
• Angular reconstitution
• Alignment refinements
• Projection matching
• 3D resolution criteria
• 3D refinements
• Refining towards different 3D structures simultaneously ("4D" analysis)
• 3D fitting of known X-ray structures (Chimera, Coot)
• Parallel processing
• Using image processing software (IMAGIC, Frealign)