Derivation and Culture of Human Induced Pluripotent Stem Cells (hiPSCs 2016)

Derivation and Culture of Human Induced Pluripotent Stem Cells (hiPSCs 2016) is a conference dedicated to current theory and best practices with regard to derivation and culture of human induced pluripotent stem cells (hiPSCs).

Topics

• Key advantages, disadvantages and critical elements of different approaches to somatic cell reprogramming
• Key principles of good cell culture practice (GCCP) and aseptic processing including the role of antibiotics
• Critical aspects for successful preservation and storage
• Undifferentiated, fully reprogrammed iPSCs compared to partially reprogrammed iPSC colonies
• Undifferentiated morphology of confluent iPSCs ready for passaging
• Evaluate the recovery of iPSCs and identify viable cells
• The importance of core QC and potential consequences of failing to carry it out
• Critical aspects in the passaging and maintenance of undifferentiated iPSCs
• Different capabilities of each method of determining potential pluripotency