Mouse Models: Genetics Breeding and Experimental Design 2016

Mouse Models: Genetics Breeding and Experimental Design 2016 is a course dedicated to designing, undertaking analysis and reporting experiments using mice.

Topics

• Gene & strain nomenclature
• Overview of mouse genetics & strains
• Breeding strategies for maintaining inbred, outbred and genetically altered lines
• Aspects of good colony management
• Conditional and inducible transgenesis
• Establishing and maintaining transgenic lines and gene targeted lines
• Genotyping
• CRISPR/Cas9 and emerging technologies for generating GA colonies
• Workshops on designing breeding programs
• Quality control strategies for mouse lines
• Electronic tools for colony management
• Cryopreservation
• Pilot experiments
• Experimental design:
  • The experimental unit
  • Randomisation and blinding
  • The 3Is
  • Calculating experimental numbers
  • Experimental controls
  • Ethics and the 3Rs
  • Standardisation and variability: effects of the environment, time and health status on experimental results
  • Statistical analysis